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All samples were serially diluted in assay buffer in duplicate and incubated with the cells for 30 min at room temperature (RT) before detection with the cAMP dynamic 2 HTRF assay as described previously 72
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Here, GIP(3-30)NH 2 and GIP(5-30)NH 2 were characterized as potent, competitive and high-affinity antagonists of the human GIP receptor ( Table 1 )
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